Cloned (Comment) | Organism |
---|---|
PCR product inserted into pQE30 vector and expressed in Escherichia coli XL1-Blue | Sinorhizobium meliloti 1021 |
Protein Variants | Comment | Organism |
---|---|---|
S10A | completely loses activity | Sinorhizobium meliloti 1021 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Sinorhizobium meliloti 1021 | |
ethanol | with 20% ethanol and 5-10% sodium dodecyl sulfate, whereas no significant changes in presence of 10% ethanol. Mg2+ and Ca2+ have no effect | Sinorhizobium meliloti 1021 | |
Urea | leads to complete unfolding at 2.0 M | Sinorhizobium meliloti 1021 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22900 | - |
3 * 22900, sequence analysis | Sinorhizobium meliloti 1021 |
69550 | - |
dynamic light scattering | Sinorhizobium meliloti 1021 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti 1021 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by sonication and on Ni-NTA affinity resin | Sinorhizobium meliloti 1021 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
recombinant Sm23, with 4-nitrophenyl acetate as substrate | Sinorhizobium meliloti 1021 |
Storage Stability | Organism |
---|---|
-20°C | Sinorhizobium meliloti 1021 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | shows maximum activity (100%) against p-nitrophenyl acetate | Sinorhizobium meliloti 1021 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Sinorhizobium meliloti 1021 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | low activity | Sinorhizobium meliloti 1021 | 4-nitrophenol + decanoate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Sinorhizobium meliloti 1021 | alpha-naphthol + acetate | - |
? | |
beta-naphthyl acetate + H2O | - |
Sinorhizobium meliloti 1021 | beta-naphthol + acetate | - |
? | |
additional information | can not hydrolyze organophosphates | Sinorhizobium meliloti 1021 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 22900, sequence analysis | Sinorhizobium meliloti 1021 |
Synonyms | Comment | Organism |
---|---|---|
SGNH-arylesterase | - |
Sinorhizobium meliloti 1021 |
Sm23 | - |
Sinorhizobium meliloti 1021 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
is resistant to thermal denaturation up to 50°C. Sm23 irreversibly unfolds after heating over 60°C | Sinorhizobium meliloti 1021 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sinorhizobium meliloti 1021 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
at pH 4-6 it is highly unstable and disordered, less than 40% of its maximal activity at pH 7.0 | Sinorhizobium meliloti 1021 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sinorhizobium meliloti 1021 | - |
- |
4.89 |
General Information | Comment | Organism |
---|---|---|
physiological function | contains a catalytic triad (Ser10, Asp187, and His190) and oxyanion holes (Ser10-Gly50-Asn90). Spherical or globular aggregates are formed with 1-butyl-3-methylimidazolium tetrafluoroborate, while amorphous aggregates are formed with 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide | Sinorhizobium meliloti 1021 |